![]() ![]() “There is no difference in Protein X concentrations from the media of peripheral blood mononuclear cells extracted from controls and those with rheumatoid arthritis.” We want to know if there is a significant difference in Protein X concentrations between the control and rheumatoid arthritis cells after 24-hours. We performed an enzyme linked-immunosorbent assay (ELISA) on the media after 24-hours to determine the concentration of Protein X. ![]() For instance, say we have performed a cell culture experiment where we incubated peripheral blood mononuclear cells extracted from controls and individuals who suffered from rheumatoid arthritis. Let’s perform a Mann-Whitney U test on some example data. How to perform a Mann-Whitney U test in SPSS The distributions of the two variables must be similar in shape.The dependent variable should be measured in two independent (non-related) groups.The dependent variable should be measured on an ordinal or continuous scale.There are just three assumptions that the data has to pass before performing a Mann-Whitney U test in SPSS. The reason you would perform a Mann-Whitney U test over an independent t-test is when the data is not normally distributed. A Mann-Whitney U test is a non-parametric alternative to the independent (unpaired) t-test to determine the difference between two groups of either continuous or ordinal data. ![]()
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